STAPHYLOCOCCAL ENTEROTOXIN B2 · Experiments

Pipeline experiments

Planned and available for reproduction, protocols and order files are included.

TR-FRETAvailable

IVTT TR-FRET screen — STAPHYLOCOCCAL_ENTEROTOXIN_B2 gBlock constructs

Campaign: staphylococcal-enterotoxin-b2--seb-01-all-generators

96-well IDT gBlocks plate. Construct: T7 promoter + GG + SD + Kozak + ATG + FLAG (DYKDDDDK) + E. coli MFC CDS + TAATAG (2x stop) + GTCGAC + T7 terminator (SOP 03). QC: 11/11 valid per gblock_qc.tsv.

Procedure

Vector: linear gBlock template (T7-SD-Kozak-FLAG construct)

IDT gBlocks (96-well plate)
TnT T7 Quick IVTT kit
HTRF anti-FLAG reagents

codon_table: E. coli MFC
construct_context: T7+GG+SD+Kozak+ATG+FLAG+CDS+TAATAG+GTCGAC+T7-terminator

Order files

staphylococcal-enterotoxin-b2--seb-01-all-generators_gblocks_plate.xlsx

Candidates (11)

Position	Candidate	Sequence	Tool	Unified score
A1	SEB_01_All_Generators_pxdesign_binder_001	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGAGCAAATATGAAAAAGCGAAAGAACTGGCGGAAAAAAAAGGCGAAGAACTGGATAAAAAAATTAAAGAACTGTATGCGAAAGGCAAAAAAATTACCAGCGATGAACGTCGTAAAATGGCGCAGGAAGTGCGTAAAGAAGTGGATAAAGAAGTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	pxdesign	0.80
A2	SEB_01_All_Generators_bindcraft_binder_006	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGCGTAGCATTGAACCGATTAAAGAAGAACTGGAATATCATCTGGAACGTAGCATTAAACATAAAATGCCGGTGGAAGAATTTAAAAAAGAACTGGAAGTGCTGAAACGTCATGTGGAATATTATTATAGCGAAGAAGAAGTGGAAGAATTTGAAAAAAAAGTGAAAGAACTGCTGGAAAAAAAAAAAAAATATGAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	bindcraft	0.72
A3	SEB_01_All_Generators_bindcraft_binder_005	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGCGTAGCATTGAACCGATTTTTGAAGAAGCGGAATATCATTTTGAACGTGCGAAAAAACATAAAATGCCGGTGGAAGAATTTGAAAAAGAACTGGAAGTGCTGATGCGTCATGTGGAATATTATTATAGCGAAGAAGTGGTGAAAAAATTTAAAGAAAAAGTGAAAGAACTGCTGGAAGAACATAAAAAACTGGAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	bindcraft	0.72
A4	SEB_01_All_Generators_bindcraft_binder_009	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGATGAAACCGACCATGCAGGAACTGCTGATTATGAACGCGAAAGAAATTGCGGATTATTATCTGAACGTGCTGACCACCAAAGAAATGACCGAAGAACAGAAAAAACATTATAAAGAAGAATTTACCAAAAGCCTGGATTATATTCTGTATGTGGCGAAAGTGCATGAATTTGATGTGGAAGAAATTGAAAAAGAAGTGGAAAAACTGAAAAAAAAAGTGCTGAGCTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	bindcraft	0.71
A5	SEB_01_All_Generators_bindcraft_binder_001	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGATGCTGAGCCCGAACGAAGTGAAAGCGCTGGAACTGATTAGCAAACTGAGCAAAGAACATCAGGAAGGCCTGAAAATGATTATTGATACCCATCGTGAATATTTTAAAGATACCGGCGAAGATGAAGATGCGTTTTTTAAACAGTTTGTGGAAGATATTGAAAAAAAATTTGGCGTGAAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	bindcraft	0.71
A6	SEB_01_All_Generators_bindcraft_binder_004	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGATGACCGATGAAGAATTTGTGATGGGCCATGTGAAAGCGGCGAAAGAAATGCTGGAAGAAACCAAAAAAAACGATCCGGAAAACTATGAAAAAGATCGTAAAGTGTATATTGAATATGTGCAGTTTCTGATTGATCATTATGCGCCGGATCTGCCGCGTACCGCGGAAGAACTGCTGAGCTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	bindcraft	0.71
A7	SEB_01_All_Generators_bindcraft_binder_003	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGATGACCGATGAAGAATTTGTGCTGGGCCATGTGAAAGCGGCGAAAGAAATGCTGGAAGAAACCAAAAAAAACGATCCGGAAAACTATGAACGTGATAAAAAAGTGTATATTGAATATGTGCAGTTTCTGATTGATCATTATGCGCCGGATCTGCCGCGTAGCGCGGAAGAACTGCTGGCGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	bindcraft	0.70
A8	SEB_01_All_Generators_bindcraft_binder_002	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGATGCTGAGCGAAGAAGAAAAAAAATTTCTGGAACTGGTGAGCAAACTGAGCGAAGAACATCAGAAAGGCCTGAAAATGATTCTGGATACCCATAAAGAATATTTTAAAGATACCGGCGAAAACGAAGATGAATTTTTTAAACAGTTTGTGGAAGATGTGAAAGAAAAATTTGGCATTGAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	bindcraft	0.70
A9	SEB_01_All_Generators_boltzgen_binder_001	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGAAAGAAAAAGCGGAAAAAGCGGCGGAAGAAGCGGCGAAAGAAGGCAAAGAACTGGGCCTGAAAGAACTGGAACGTCTGCATGCGGAACATCCGGAACGTGTGGATCTGGAAGAAGCGAAAAAAGAACTGGAAAAAAACCTGGAAGCGGGCAAAAAATATATTGTGGAAAGCCTGGTGAAACTGGAAAAAGAACGTAAAGAAAACTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.70
A10	SEB_01_All_Generators_bindcraft_binder_008	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGAAAAAAGTGAGCGTGGAAGAATTTAAAGATATTGTGATGGCGCTGCTGTATACCTATGGCTTTGATATGAGCGATGAAGAAAAAAAAGAAATTTTTGATAAAGTGATGGAAATTGCGAAAACCGAAGGCGTGGAAAAAGCGTGGGAAGAATTTAAAAAAGAAATGAAAAAATATGTGCCGGAATTTCCGGAATATATTCTGAACACCCCGATTGAACAGATGATGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	bindcraft	0.67
A11	SEB_01_All_Generators_bindcraft_binder_007	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGAAAAAAGTGAGCGTGGAAGAATTTGAAGATATTGTGATGGCGCTGCTGTATACCTATGGCTTTGATATGAGCGAAGAAGAAAAAGAAAAAATTTATAAAGAAGTGATGGAAATTGCGAAAACCGAAGGCGTGGAAAAAGCGTGGGAACTGTTTAAAGAAAAAATGAAAAAATATGTGCCGGAATTTCCGGAATATATTCTGAACACCCCGATTGAACAGATGATGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	bindcraft	0.67

Yeast Surface DisplayAvailable

Yeast surface display screen — STAPHYLOCOCCAL_ENTEROTOXIN_B2 binder oligo pools

Campaign: staphylococcal-enterotoxin-b2--seb-01-all-generators

IDT oPools order, tiered by confidence: tier1_50pmol (29 oligos). Gap-repair cloning into pCTCON2.

Procedure

Vector: pCTCON2

IDT oPools (tiered by confidence)
pCTCON2 (gap-repair linearized)

Order files

Candidates (29 · tier 1)

Position	Candidate	Sequence	Tool	Pool score
tier1_50pmol	SEB_01_All_Generators_bindcraft_binder_003	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGATGACTGATGAAGAATTCGTTTTGGGTCACGTTAAAGCTGCTAAAGAAATGTTGGAAGAAACTAAAAAAAACGATCCAGAAAACTACGAAAGAGATAAAAAAGTTTACATTGAATACGTTCAATTCTTGATTGATCACTACGCTCCAGATTTGCCAAGATCTGCTGAAGAATTGTTGGCTGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	bindcraft	0.88
tier1_50pmol	SEB_01_All_Generators_bindcraft_binder_004	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGATGACTGATGAAGAATTCGTTATGGGTCACGTTAAAGCTGCTAAAGAAATGTTGGAAGAAACTAAAAAAAACGATCCAGAAAACTACGAAAAAGATAGAAAAGTTTACATTGAATACGTTCAATTCTTGATTGATCACTACGCTCCAGATTTGCCAAGAACTGCTGAAGAATTGTTGTCTGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	bindcraft	0.88
tier1_50pmol	SEB_01_All_Generators_bindcraft_binder_001	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGATGTTGTCTCCAAACGAAGTTAAAGCTTTGGAATTGATTTCTAAATTGTCTAAAGAACACCAAGAAGGTTTGAAAATGATTATTGATACTCACAGAGAATACTTCAAAGATACTGGTGAAGATGAAGATGCTTTCTTCAAACAATTCGTTGAAGATATTGAAAAAAAATTCGGTGTTAAAGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	bindcraft	0.86
tier1_50pmol	SEB_01_All_Generators_bindcraft_binder_009	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGATGAAACCAACTATGCAAGAATTGTTGATTATGAACGCTAAAGAAATTGCTGATTACTACTTGAACGTTTTGACTACTAAAGAAATGACTGAAGAACAAAAAAAACACTACAAAGAAGAATTCACTAAATCTTTGGATTACATTTTGTACGTTGCTAAAGTTCACGAATTCGATGTTGAAGAAATTGAAAAAGAAGTTGAAAAATTGAAAAAAAAAGTTTTGTCTGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	bindcraft	0.85
tier1_50pmol	SEB_01_All_Generators_bindcraft_binder_005	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGAGATCTATTGAACCAATTTTCGAAGAAGCTGAATACCACTTCGAAAGAGCTAAAAAACACAAAATGCCAGTTGAAGAATTCGAAAAAGAATTGGAAGTTTTGATGAGACACGTTGAATACTACTACTCTGAAGAAGTTGTTAAAAAATTCAAAGAAAAAGTTAAAGAATTGTTGGAAGAACACAAAAAATTGGAAGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	bindcraft	0.83
tier1_50pmol	SEB_01_All_Generators_bindcraft_binder_007	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGAAAAAAGTTTCTGTTGAAGAATTCGAAGATATTGTTATGGCTTTGTTGTACACTTACGGTTTCGATATGTCTGAAGAAGAAAAAGAAAAAATTTACAAAGAAGTTATGGAAATTGCTAAAACTGAAGGTGTTGAAAAAGCTTGGGAATTGTTCAAAGAAAAAATGAAAAAATACGTTCCAGAATTCCCAGAATACATTTTGAACACTCCAATTGAACAAATGATGGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	bindcraft	0.83
tier1_50pmol	SEB_01_All_Generators_bindcraft_binder_002	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGATGTTGTCTGAAGAAGAAAAAAAATTCTTGGAATTGGTTTCTAAATTGTCTGAAGAACACCAAAAAGGTTTGAAAATGATTTTGGATACTCACAAAGAATACTTCAAAGATACTGGTGAAAACGAAGATGAATTCTTCAAACAATTCGTTGAAGATGTTAAAGAAAAATTCGGTATTGAAGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	bindcraft	0.83
tier1_50pmol	SEB_01_All_Generators_bindcraft_binder_008	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGAAAAAAGTTTCTGTTGAAGAATTCAAAGATATTGTTATGGCTTTGTTGTACACTTACGGTTTCGATATGTCTGATGAAGAAAAAAAAGAAATTTTCGATAAAGTTATGGAAATTGCTAAAACTGAAGGTGTTGAAAAAGCTTGGGAAGAATTCAAAAAAGAAATGAAAAAATACGTTCCAGAATTCCCAGAATACATTTTGAACACTCCAATTGAACAAATGATGGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	bindcraft	0.82
tier1_50pmol	SEB_01_All_Generators_bindcraft_binder_006	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGAGATCTATTGAACCAATTAAAGAAGAATTGGAATACCACTTGGAAAGATCTATTAAACACAAAATGCCAGTTGAAGAATTCAAAAAAGAATTGGAAGTTTTGAAAAGACACGTTGAATACTACTACTCTGAAGAAGAAGTTGAAGAATTCGAAAAAAAAGTTAAAGAATTGTTGGAAAAAAAAAAAAAATACGAAGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	bindcraft	0.80
tier1_50pmol	SEB_01_All_Generators_pxdesign_binder_001	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGTCTAAATACGAAAAAGCTAAAGAATTGGCTGAAAAAAAAGGTGAAGAATTGGATAAAAAAATTAAAGAATTGTACGCTAAAGGTAAAAAAATTACTTCTGATGAAAGAAGAAAAATGGCTCAAGAAGTTAGAAAAGAAGTTGATAAAGAAGTTGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	pxdesign	0.78
tier1_50pmol	SEB_01_All_Generators_pxdesign_binder_004	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGTCTATTGAAGAAAAAGTTAAAGAATACCAAGAAAAATTGGCTGAAGAAGTTTTGAAAGAAATTGAAAAAATTGCTAAAGAAAAAAAATTGTCTGATGAAGAAATTAACGAATTGAGAAGAAAAAAAGGTACTGAATTGACTTTGGAAATTGATAAATACTACAAAAAATTGAAAGAAGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	pxdesign	0.73
tier1_50pmol	SEB_01_All_Generators_pxdesign_binder_002	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGTCTCAAAAAGAATTGGCTGAAAAAAAATTGGAAGAATTCAAAAAATTGTTGGAAGAAAACAAAAACTTGCCAAAAGATGAATTCGATAAAAAATTCAACGAAAAAATTAACGAAATTAAAAAATACTTCGTTGAAAACAACATTTCTGATGAAATTTTCAAAGAATTGATGGAAAAATTGGAAGAATTGTACGAAGAAAAATTGAAAGAAGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	pxdesign	0.69
tier1_50pmol	SEB_01_All_Generators_pxdesign_binder_006	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGTCTGAAGAAAAAAAATTGGAATCTTTGAAAGAAGCTGCTAAAACTTTGAAAAAATTGATTAAAAACTTGAAAAAAATTGCTTCTGAAGAAACTAAACCATTGGTTGAAAAAGCTGAAGCTAAATTGAAAGAAGCTGAAGCTTTGATTAAAGCTAAAGCTCCATTGGAAGAAATTGAAAAAAAATTGGAAGAAGCTTACGCTTACGCTAACAGAGCTGCTGAATTGGTTTTGTCTGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	pxdesign	0.58
tier1_50pmol	SEB_01_All_Generators_pxdesign_binder_005	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGTCTTTGGAAGAATACCAAGAAAAAGGTGAAGAATTGTACAACGAAGAATTGAAAAAATTGATTAAAGAAGCTAAAGAAAACAACTTGTCTGATGAAGAATTCATTAACAAATTGAGAAAAAAAGCTCAAGAAATTAACTCTAAATTGGAAAAAGAATACGAAGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	pxdesign	0.58
tier1_50pmol	SEB_01_All_Generators_pxdesign_binder_008	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGTCTTTGGAAGAATTGGAAGAAGAATTGCAAAAAGTTTTGGATGAAATTAACAAAGTTTACTCTTTGATTATGAAAAACGGTTCTGAAGAAGATAAAAAAGAAGCTACTGAATTGTTCAACGAAGTTAACAAAGCTTTGCAAGAAGCTAAAGAAAAAAACACTGAAGAAGCTGCTAAAGAAGCTTTGAAAAAAGCTAAAGAAAAATTGAAAAAATTGAAAGAAATTTTGGAAAAAGCTTTGGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	pxdesign	0.56
tier1_50pmol	SEB_01_All_Generators_pxdesign_binder_007	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGTCTGAAAACATGAAAAAAGCTGTTGAAGAAATTGCTAAAGTTTACGATGAATTGTTGAAAAAATACTTCAACGGTGAATTGACTGAAGAAGAAGCTATTAAAGAATTGGAAGAAAAAACTGAAGAAATTGCTAAAAAATACAACATTCCAGAAGAAGATGCTAAATCTTTGAAAATTACTGCTGTTTCTGGTTTCAAAAGAGATGTTGAAACTTACAAAAAAGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	pxdesign	0.56
tier1_50pmol	SEB_01_All_Generators_boltzgen_binder_002	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGCAAGTTGAAGCTATGAAAAAAGCTTACGAAGCTTTGAAAGCTCAAGGTTACACTCCATCTTCTAAAATTTCTATTAAAGAAGTTTTGGATACTGTTAAAGCTGCTGGTGCTGAAGTTGATGAAGAAAAATTGGAAGAAAACTTGAAAAAAGCTGGTTTGACTTTGGATTCTTTGGTTTCTGTTGGTACTGCTTTGGCTTTGGTTGGTGCTGCTTTGGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	boltzgen	0.55
tier1_50pmol	SEB_01_All_Generators_pxdesign_binder_010	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGTCTTTGGAAGAAGCTAAAAAAGAAGTTGAAAAAATTGTTGAAGAAAAATTGCCAGAATTGATTGAAGCTGATAAAAAAAAAGATGTTGAAGAAGCTAAAAAAATTGTTACTGAAATTTCTGATAAAGTTGTTGAAATTGGTAAAAAAAACAACTTGTCTTACGAAGAAATTAACGAATTGGTTAGAGAAGCTATTACTGAAATTTACAACAAAGTTGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	pxdesign	0.55
tier1_50pmol	SEB_01_All_Generators_pxdesign_binder_009	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGTCTTCTAAAGAAGATTTGGAAAAATTGGAAGAATTGGTTAAAGAAATTAAAGCTGATCCATCTAAAGCTACTGATGAAGTTATTGATGAAATTAAAGAATTGGCTAACAGAATTTACAAAAACGGTACTAAAGAAGAACAAAGAAGAGCTGCTAAATTGGTTGGTGAATTCGCTGAAATTGTTCACAAATTGAAAAAAAAAAAAGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	pxdesign	0.54
tier1_50pmol	SEB_01_All_Generators_pxdesign_binder_003	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGATGTCTAAAGAAGAAAAATTGAAAGAAGCTAAAGAAAAAGTTGCTGAAGAAGGTTACGAAAAATTGAAAGAATTCATTAAAAAAAACGGTGAAAAAATTACTGAAGAAGAATTCATTAAAGAAAACGCTAAAATTGGTAACGAACAATTCAAAAAATTCGAAGAAGAAGCTAAAAAAATTGAAAAAGAAGAAAAAGAAGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	pxdesign	0.53
tier1_50pmol	SEB_01_All_Generators_boltzgen_binder_001	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGAAAGAAAAAGCTGAAAAAGCTGCTGAAGAAGCTGCTAAAGAAGGTAAAGAATTGGGTTTGAAAGAATTGGAAAGATTGCACGCTGAACACCCAGAAAGAGTTGATTTGGAAGAAGCTAAAAAAGAATTGGAAAAAAACTTGGAAGCTGGTAAAAAATACATTGTTGAATCTTTGGTTAAATTGGAAAAAGAAAGAAAAGAAAACGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	boltzgen	0.52
tier1_50pmol	SEB_01_All_Generators_boltzgen_binder_004	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGATGTTGGTTGAAGTTCCAAGAGAAAACAGAAGAGAATTGTTGGCTGAAATTGTTGAATTGTACGAAGAAACTGGTGATGAAGTTTTGAGAAACTTGGCTAGACAAGTTATTGAAACTGGTGTTGTTAACACTGATTTGTTGGATGAATTGCCAGAAGAAATTAGAGAAAAAGTTTTGGAATTGATTGAAAAATACGCTGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	boltzgen	0.37
tier1_50pmol	SEB_01_All_Generators_boltzgen_binder_003	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGGCTAAAAGAGAAGCTGCTTTGAAAGCTGTTAAAGAAGCTATTGAAAAATCTGGTGGTTCTATTGAAAGAGGTACTGTTGCTGAAATTTTGAAAGAACACTTGGGTTTGTCTGATGAAACTATTGCTGAAGCTTACAGAGCTTTGGGTTTGCCATCTGTTTTGAACTTGGAAGAAGCTGAAGCTTTGGTTGATGCTGCTACTGCTGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	boltzgen	0.29
tier1_50pmol	SEB_01_All_Generators_boltzgen_binder_006	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGGAAGAAGCTAGAAAAAAAGCTGCTGAATTGTTGAAAAAAGCTGATGAATTGGCTTTGAAAGGTAAAGGTGATGAAGCTTTGGCTGCTGGTAGAGAAGCTGCTGCTTTGGCTGCTGCTGAAGATCCAGCTGCTGCTGGTTTCGCTAGAGCTAGAGGTGAATTGTTGAAAGAAGCTGCTGAAGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	boltzgen	0.28
tier1_50pmol	SEB_01_All_Generators_boltzgen_binder_008	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGGGTGCTGCTGCTGCTGCTGGTAGAGCTAAAGGTCAAGAAATTAGAGATAAATACGATAAAGAAGCTAACAAATTGGCTAAAGAATTGGAAGGTGAAGAAGCTGATAAAGCTGCTGAAGAATTGGCTGCTGAATACGGTAAAGAAGCTGCTGCTGCTAACGGTAAAATTGGTGAAGCTGCTAAAGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	boltzgen	0.28
tier1_50pmol	SEB_01_All_Generators_boltzgen_binder_005	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGATGATTACTTTGGTTTTCGATGATGGTAAAAAAGTTGAAGTTAAATCTCAAGAAGAATTGTTGGAAGAAATTAAAGTTTTGGGTTTGCCAGAAGAATGCTTGGAATTGACTTACAACGGTAAAACTTTCACTACTTTGGAAGAATTCAAAAAAGTTAAAATTAACGATGGTGATACTGTTAACTTGAAATTGAAATTGCCAGTTGAAGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	boltzgen	0.27
tier1_50pmol	SEB_01_All_Generators_boltzgen_binder_007	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGATGCAAATTTTCGTTAAAACTACTACTGGTAAAACTATTACTTTGGAAGTTGAACCATCTGATACTGTTGGTAACGTTAAAGCTAAAATTCAAGCTATTGAACCATTCGAAGAATTGATTTTCGCTGGTAAACAATTGGAAGATGGTAGAACTTTGTCTGATTACAACATTCAAAAAGAATCTACTTTGCACTTGGTTTTGCCAGGTGAATACGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	boltzgen	0.27
tier1_50pmol	SEB_01_All_Generators_boltzgen_binder_009	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGGCTGCTGGTGCTGCTGCTGGTGCTGCTGGTGGTGCTGCTGGTTCTGCTTCTGCTGCTGGTGCTGGTGCTGGTGCTGGTGGTGGTGAAGGTGGTGCTGCTGGTGCTGCTGCTGGTGGTGCTGCTGGTGCTGCTGCTGGTGCTGCTGCTGGTTCTGCTGCTGCTGCTGCTGGTGCTTTGGGTGAATTGGCTGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	boltzgen	0.26
tier1_50pmol	SEB_01_All_Generators_boltzgen_binder_010	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGGAAAAAAGAGAAAAAATTATTGAATTGTTGTTGGAATTCGGTTTCTCTAAAGAAGAAGCTGAAGAAATTGCTGAAAGATTGTTGGAATTGTACAAAAAAGGTACTTTGAACTTGGCTGCTGTTACTGAATTGTTGGCTGAATTCAAAAAATTGTCTAAAGAAGAAGTTGAAGAAATTTACAAAAGAATTTTGGAAATTTTGTCTGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	boltzgen	0.26

Pipeline experiments

Planned and available for reproduction, protocols and order files are included.

TR-FRETAvailable

IVTT TR-FRET screen — STAPHYLOCOCCAL_ENTEROTOXIN_B2 gBlock constructs

Campaign: staphylococcal-enterotoxin-b2--seb-01-all-generators

96-well IDT gBlocks plate. Construct: T7 promoter + GG + SD + Kozak + ATG + FLAG (DYKDDDDK) + E. coli MFC CDS + TAATAG (2x stop) + GTCGAC + T7 terminator (SOP 03). QC: 11/11 valid per gblock_qc.tsv.

Procedure

Vector: linear gBlock template (T7-SD-Kozak-FLAG construct)

IDT gBlocks (96-well plate)
TnT T7 Quick IVTT kit
HTRF anti-FLAG reagents

codon_table: E. coli MFC
construct_context: T7+GG+SD+Kozak+ATG+FLAG+CDS+TAATAG+GTCGAC+T7-terminator

Order files

staphylococcal-enterotoxin-b2--seb-01-all-generators_gblocks_plate.xlsx

Candidates (11)

Position	Candidate	Sequence	Tool	Unified score
A1	SEB_01_All_Generators_pxdesign_binder_001	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGAGCAAATATGAAAAAGCGAAAGAACTGGCGGAAAAAAAAGGCGAAGAACTGGATAAAAAAATTAAAGAACTGTATGCGAAAGGCAAAAAAATTACCAGCGATGAACGTCGTAAAATGGCGCAGGAAGTGCGTAAAGAAGTGGATAAAGAAGTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	pxdesign	0.80
A2	SEB_01_All_Generators_bindcraft_binder_006	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGCGTAGCATTGAACCGATTAAAGAAGAACTGGAATATCATCTGGAACGTAGCATTAAACATAAAATGCCGGTGGAAGAATTTAAAAAAGAACTGGAAGTGCTGAAACGTCATGTGGAATATTATTATAGCGAAGAAGAAGTGGAAGAATTTGAAAAAAAAGTGAAAGAACTGCTGGAAAAAAAAAAAAAATATGAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	bindcraft	0.72
A3	SEB_01_All_Generators_bindcraft_binder_005	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGCGTAGCATTGAACCGATTTTTGAAGAAGCGGAATATCATTTTGAACGTGCGAAAAAACATAAAATGCCGGTGGAAGAATTTGAAAAAGAACTGGAAGTGCTGATGCGTCATGTGGAATATTATTATAGCGAAGAAGTGGTGAAAAAATTTAAAGAAAAAGTGAAAGAACTGCTGGAAGAACATAAAAAACTGGAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	bindcraft	0.72
A4	SEB_01_All_Generators_bindcraft_binder_009	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGATGAAACCGACCATGCAGGAACTGCTGATTATGAACGCGAAAGAAATTGCGGATTATTATCTGAACGTGCTGACCACCAAAGAAATGACCGAAGAACAGAAAAAACATTATAAAGAAGAATTTACCAAAAGCCTGGATTATATTCTGTATGTGGCGAAAGTGCATGAATTTGATGTGGAAGAAATTGAAAAAGAAGTGGAAAAACTGAAAAAAAAAGTGCTGAGCTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	bindcraft	0.71
A5	SEB_01_All_Generators_bindcraft_binder_001	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGATGCTGAGCCCGAACGAAGTGAAAGCGCTGGAACTGATTAGCAAACTGAGCAAAGAACATCAGGAAGGCCTGAAAATGATTATTGATACCCATCGTGAATATTTTAAAGATACCGGCGAAGATGAAGATGCGTTTTTTAAACAGTTTGTGGAAGATATTGAAAAAAAATTTGGCGTGAAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	bindcraft	0.71
A6	SEB_01_All_Generators_bindcraft_binder_004	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGATGACCGATGAAGAATTTGTGATGGGCCATGTGAAAGCGGCGAAAGAAATGCTGGAAGAAACCAAAAAAAACGATCCGGAAAACTATGAAAAAGATCGTAAAGTGTATATTGAATATGTGCAGTTTCTGATTGATCATTATGCGCCGGATCTGCCGCGTACCGCGGAAGAACTGCTGAGCTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	bindcraft	0.71
A7	SEB_01_All_Generators_bindcraft_binder_003	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGATGACCGATGAAGAATTTGTGCTGGGCCATGTGAAAGCGGCGAAAGAAATGCTGGAAGAAACCAAAAAAAACGATCCGGAAAACTATGAACGTGATAAAAAAGTGTATATTGAATATGTGCAGTTTCTGATTGATCATTATGCGCCGGATCTGCCGCGTAGCGCGGAAGAACTGCTGGCGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	bindcraft	0.70
A8	SEB_01_All_Generators_bindcraft_binder_002	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGATGCTGAGCGAAGAAGAAAAAAAATTTCTGGAACTGGTGAGCAAACTGAGCGAAGAACATCAGAAAGGCCTGAAAATGATTCTGGATACCCATAAAGAATATTTTAAAGATACCGGCGAAAACGAAGATGAATTTTTTAAACAGTTTGTGGAAGATGTGAAAGAAAAATTTGGCATTGAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	bindcraft	0.70
A9	SEB_01_All_Generators_boltzgen_binder_001	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGAAAGAAAAAGCGGAAAAAGCGGCGGAAGAAGCGGCGAAAGAAGGCAAAGAACTGGGCCTGAAAGAACTGGAACGTCTGCATGCGGAACATCCGGAACGTGTGGATCTGGAAGAAGCGAAAAAAGAACTGGAAAAAAACCTGGAAGCGGGCAAAAAATATATTGTGGAAAGCCTGGTGAAACTGGAAAAAGAACGTAAAGAAAACTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.70
A10	SEB_01_All_Generators_bindcraft_binder_008	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGAAAAAAGTGAGCGTGGAAGAATTTAAAGATATTGTGATGGCGCTGCTGTATACCTATGGCTTTGATATGAGCGATGAAGAAAAAAAAGAAATTTTTGATAAAGTGATGGAAATTGCGAAAACCGAAGGCGTGGAAAAAGCGTGGGAAGAATTTAAAAAAGAAATGAAAAAATATGTGCCGGAATTTCCGGAATATATTCTGAACACCCCGATTGAACAGATGATGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	bindcraft	0.67
A11	SEB_01_All_Generators_bindcraft_binder_007	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGAAAAAAGTGAGCGTGGAAGAATTTGAAGATATTGTGATGGCGCTGCTGTATACCTATGGCTTTGATATGAGCGAAGAAGAAAAAGAAAAAATTTATAAAGAAGTGATGGAAATTGCGAAAACCGAAGGCGTGGAAAAAGCGTGGGAACTGTTTAAAGAAAAAATGAAAAAATATGTGCCGGAATTTCCGGAATATATTCTGAACACCCCGATTGAACAGATGATGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	bindcraft	0.67

Yeast Surface DisplayAvailable

Yeast surface display screen — STAPHYLOCOCCAL_ENTEROTOXIN_B2 binder oligo pools

Campaign: staphylococcal-enterotoxin-b2--seb-01-all-generators

IDT oPools order, tiered by confidence: tier1_50pmol (29 oligos). Gap-repair cloning into pCTCON2.

Procedure

Vector: pCTCON2

IDT oPools (tiered by confidence)
pCTCON2 (gap-repair linearized)

Order files

Candidates (29 · tier 1)

Position	Candidate	Sequence	Tool	Pool score
tier1_50pmol	SEB_01_All_Generators_bindcraft_binder_003	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGATGACTGATGAAGAATTCGTTTTGGGTCACGTTAAAGCTGCTAAAGAAATGTTGGAAGAAACTAAAAAAAACGATCCAGAAAACTACGAAAGAGATAAAAAAGTTTACATTGAATACGTTCAATTCTTGATTGATCACTACGCTCCAGATTTGCCAAGATCTGCTGAAGAATTGTTGGCTGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	bindcraft	0.88
tier1_50pmol	SEB_01_All_Generators_bindcraft_binder_004	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGATGACTGATGAAGAATTCGTTATGGGTCACGTTAAAGCTGCTAAAGAAATGTTGGAAGAAACTAAAAAAAACGATCCAGAAAACTACGAAAAAGATAGAAAAGTTTACATTGAATACGTTCAATTCTTGATTGATCACTACGCTCCAGATTTGCCAAGAACTGCTGAAGAATTGTTGTCTGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	bindcraft	0.88
tier1_50pmol	SEB_01_All_Generators_bindcraft_binder_001	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGATGTTGTCTCCAAACGAAGTTAAAGCTTTGGAATTGATTTCTAAATTGTCTAAAGAACACCAAGAAGGTTTGAAAATGATTATTGATACTCACAGAGAATACTTCAAAGATACTGGTGAAGATGAAGATGCTTTCTTCAAACAATTCGTTGAAGATATTGAAAAAAAATTCGGTGTTAAAGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	bindcraft	0.86
tier1_50pmol	SEB_01_All_Generators_bindcraft_binder_009	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGATGAAACCAACTATGCAAGAATTGTTGATTATGAACGCTAAAGAAATTGCTGATTACTACTTGAACGTTTTGACTACTAAAGAAATGACTGAAGAACAAAAAAAACACTACAAAGAAGAATTCACTAAATCTTTGGATTACATTTTGTACGTTGCTAAAGTTCACGAATTCGATGTTGAAGAAATTGAAAAAGAAGTTGAAAAATTGAAAAAAAAAGTTTTGTCTGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	bindcraft	0.85
tier1_50pmol	SEB_01_All_Generators_bindcraft_binder_005	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGAGATCTATTGAACCAATTTTCGAAGAAGCTGAATACCACTTCGAAAGAGCTAAAAAACACAAAATGCCAGTTGAAGAATTCGAAAAAGAATTGGAAGTTTTGATGAGACACGTTGAATACTACTACTCTGAAGAAGTTGTTAAAAAATTCAAAGAAAAAGTTAAAGAATTGTTGGAAGAACACAAAAAATTGGAAGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	bindcraft	0.83
tier1_50pmol	SEB_01_All_Generators_bindcraft_binder_007	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGAAAAAAGTTTCTGTTGAAGAATTCGAAGATATTGTTATGGCTTTGTTGTACACTTACGGTTTCGATATGTCTGAAGAAGAAAAAGAAAAAATTTACAAAGAAGTTATGGAAATTGCTAAAACTGAAGGTGTTGAAAAAGCTTGGGAATTGTTCAAAGAAAAAATGAAAAAATACGTTCCAGAATTCCCAGAATACATTTTGAACACTCCAATTGAACAAATGATGGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	bindcraft	0.83
tier1_50pmol	SEB_01_All_Generators_bindcraft_binder_002	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGATGTTGTCTGAAGAAGAAAAAAAATTCTTGGAATTGGTTTCTAAATTGTCTGAAGAACACCAAAAAGGTTTGAAAATGATTTTGGATACTCACAAAGAATACTTCAAAGATACTGGTGAAAACGAAGATGAATTCTTCAAACAATTCGTTGAAGATGTTAAAGAAAAATTCGGTATTGAAGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	bindcraft	0.83
tier1_50pmol	SEB_01_All_Generators_bindcraft_binder_008	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGAAAAAAGTTTCTGTTGAAGAATTCAAAGATATTGTTATGGCTTTGTTGTACACTTACGGTTTCGATATGTCTGATGAAGAAAAAAAAGAAATTTTCGATAAAGTTATGGAAATTGCTAAAACTGAAGGTGTTGAAAAAGCTTGGGAAGAATTCAAAAAAGAAATGAAAAAATACGTTCCAGAATTCCCAGAATACATTTTGAACACTCCAATTGAACAAATGATGGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	bindcraft	0.82
tier1_50pmol	SEB_01_All_Generators_bindcraft_binder_006	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGAGATCTATTGAACCAATTAAAGAAGAATTGGAATACCACTTGGAAAGATCTATTAAACACAAAATGCCAGTTGAAGAATTCAAAAAAGAATTGGAAGTTTTGAAAAGACACGTTGAATACTACTACTCTGAAGAAGAAGTTGAAGAATTCGAAAAAAAAGTTAAAGAATTGTTGGAAAAAAAAAAAAAATACGAAGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	bindcraft	0.80
tier1_50pmol	SEB_01_All_Generators_pxdesign_binder_001	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGTCTAAATACGAAAAAGCTAAAGAATTGGCTGAAAAAAAAGGTGAAGAATTGGATAAAAAAATTAAAGAATTGTACGCTAAAGGTAAAAAAATTACTTCTGATGAAAGAAGAAAAATGGCTCAAGAAGTTAGAAAAGAAGTTGATAAAGAAGTTGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	pxdesign	0.78
tier1_50pmol	SEB_01_All_Generators_pxdesign_binder_004	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGTCTATTGAAGAAAAAGTTAAAGAATACCAAGAAAAATTGGCTGAAGAAGTTTTGAAAGAAATTGAAAAAATTGCTAAAGAAAAAAAATTGTCTGATGAAGAAATTAACGAATTGAGAAGAAAAAAAGGTACTGAATTGACTTTGGAAATTGATAAATACTACAAAAAATTGAAAGAAGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	pxdesign	0.73
tier1_50pmol	SEB_01_All_Generators_pxdesign_binder_002	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGTCTCAAAAAGAATTGGCTGAAAAAAAATTGGAAGAATTCAAAAAATTGTTGGAAGAAAACAAAAACTTGCCAAAAGATGAATTCGATAAAAAATTCAACGAAAAAATTAACGAAATTAAAAAATACTTCGTTGAAAACAACATTTCTGATGAAATTTTCAAAGAATTGATGGAAAAATTGGAAGAATTGTACGAAGAAAAATTGAAAGAAGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	pxdesign	0.69
tier1_50pmol	SEB_01_All_Generators_pxdesign_binder_006	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGTCTGAAGAAAAAAAATTGGAATCTTTGAAAGAAGCTGCTAAAACTTTGAAAAAATTGATTAAAAACTTGAAAAAAATTGCTTCTGAAGAAACTAAACCATTGGTTGAAAAAGCTGAAGCTAAATTGAAAGAAGCTGAAGCTTTGATTAAAGCTAAAGCTCCATTGGAAGAAATTGAAAAAAAATTGGAAGAAGCTTACGCTTACGCTAACAGAGCTGCTGAATTGGTTTTGTCTGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	pxdesign	0.58
tier1_50pmol	SEB_01_All_Generators_pxdesign_binder_005	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGTCTTTGGAAGAATACCAAGAAAAAGGTGAAGAATTGTACAACGAAGAATTGAAAAAATTGATTAAAGAAGCTAAAGAAAACAACTTGTCTGATGAAGAATTCATTAACAAATTGAGAAAAAAAGCTCAAGAAATTAACTCTAAATTGGAAAAAGAATACGAAGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	pxdesign	0.58
tier1_50pmol	SEB_01_All_Generators_pxdesign_binder_008	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGTCTTTGGAAGAATTGGAAGAAGAATTGCAAAAAGTTTTGGATGAAATTAACAAAGTTTACTCTTTGATTATGAAAAACGGTTCTGAAGAAGATAAAAAAGAAGCTACTGAATTGTTCAACGAAGTTAACAAAGCTTTGCAAGAAGCTAAAGAAAAAAACACTGAAGAAGCTGCTAAAGAAGCTTTGAAAAAAGCTAAAGAAAAATTGAAAAAATTGAAAGAAATTTTGGAAAAAGCTTTGGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	pxdesign	0.56
tier1_50pmol	SEB_01_All_Generators_pxdesign_binder_007	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGTCTGAAAACATGAAAAAAGCTGTTGAAGAAATTGCTAAAGTTTACGATGAATTGTTGAAAAAATACTTCAACGGTGAATTGACTGAAGAAGAAGCTATTAAAGAATTGGAAGAAAAAACTGAAGAAATTGCTAAAAAATACAACATTCCAGAAGAAGATGCTAAATCTTTGAAAATTACTGCTGTTTCTGGTTTCAAAAGAGATGTTGAAACTTACAAAAAAGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	pxdesign	0.56
tier1_50pmol	SEB_01_All_Generators_boltzgen_binder_002	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGCAAGTTGAAGCTATGAAAAAAGCTTACGAAGCTTTGAAAGCTCAAGGTTACACTCCATCTTCTAAAATTTCTATTAAAGAAGTTTTGGATACTGTTAAAGCTGCTGGTGCTGAAGTTGATGAAGAAAAATTGGAAGAAAACTTGAAAAAAGCTGGTTTGACTTTGGATTCTTTGGTTTCTGTTGGTACTGCTTTGGCTTTGGTTGGTGCTGCTTTGGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	boltzgen	0.55
tier1_50pmol	SEB_01_All_Generators_pxdesign_binder_010	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGTCTTTGGAAGAAGCTAAAAAAGAAGTTGAAAAAATTGTTGAAGAAAAATTGCCAGAATTGATTGAAGCTGATAAAAAAAAAGATGTTGAAGAAGCTAAAAAAATTGTTACTGAAATTTCTGATAAAGTTGTTGAAATTGGTAAAAAAAACAACTTGTCTTACGAAGAAATTAACGAATTGGTTAGAGAAGCTATTACTGAAATTTACAACAAAGTTGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	pxdesign	0.55
tier1_50pmol	SEB_01_All_Generators_pxdesign_binder_009	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGTCTTCTAAAGAAGATTTGGAAAAATTGGAAGAATTGGTTAAAGAAATTAAAGCTGATCCATCTAAAGCTACTGATGAAGTTATTGATGAAATTAAAGAATTGGCTAACAGAATTTACAAAAACGGTACTAAAGAAGAACAAAGAAGAGCTGCTAAATTGGTTGGTGAATTCGCTGAAATTGTTCACAAATTGAAAAAAAAAAAAGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	pxdesign	0.54
tier1_50pmol	SEB_01_All_Generators_pxdesign_binder_003	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGATGTCTAAAGAAGAAAAATTGAAAGAAGCTAAAGAAAAAGTTGCTGAAGAAGGTTACGAAAAATTGAAAGAATTCATTAAAAAAAACGGTGAAAAAATTACTGAAGAAGAATTCATTAAAGAAAACGCTAAAATTGGTAACGAACAATTCAAAAAATTCGAAGAAGAAGCTAAAAAAATTGAAAAAGAAGAAAAAGAAGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	pxdesign	0.53
tier1_50pmol	SEB_01_All_Generators_boltzgen_binder_001	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGAAAGAAAAAGCTGAAAAAGCTGCTGAAGAAGCTGCTAAAGAAGGTAAAGAATTGGGTTTGAAAGAATTGGAAAGATTGCACGCTGAACACCCAGAAAGAGTTGATTTGGAAGAAGCTAAAAAAGAATTGGAAAAAAACTTGGAAGCTGGTAAAAAATACATTGTTGAATCTTTGGTTAAATTGGAAAAAGAAAGAAAAGAAAACGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	boltzgen	0.52
tier1_50pmol	SEB_01_All_Generators_boltzgen_binder_004	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGATGTTGGTTGAAGTTCCAAGAGAAAACAGAAGAGAATTGTTGGCTGAAATTGTTGAATTGTACGAAGAAACTGGTGATGAAGTTTTGAGAAACTTGGCTAGACAAGTTATTGAAACTGGTGTTGTTAACACTGATTTGTTGGATGAATTGCCAGAAGAAATTAGAGAAAAAGTTTTGGAATTGATTGAAAAATACGCTGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	boltzgen	0.37
tier1_50pmol	SEB_01_All_Generators_boltzgen_binder_003	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGGCTAAAAGAGAAGCTGCTTTGAAAGCTGTTAAAGAAGCTATTGAAAAATCTGGTGGTTCTATTGAAAGAGGTACTGTTGCTGAAATTTTGAAAGAACACTTGGGTTTGTCTGATGAAACTATTGCTGAAGCTTACAGAGCTTTGGGTTTGCCATCTGTTTTGAACTTGGAAGAAGCTGAAGCTTTGGTTGATGCTGCTACTGCTGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	boltzgen	0.29
tier1_50pmol	SEB_01_All_Generators_boltzgen_binder_006	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGGAAGAAGCTAGAAAAAAAGCTGCTGAATTGTTGAAAAAAGCTGATGAATTGGCTTTGAAAGGTAAAGGTGATGAAGCTTTGGCTGCTGGTAGAGAAGCTGCTGCTTTGGCTGCTGCTGAAGATCCAGCTGCTGCTGGTTTCGCTAGAGCTAGAGGTGAATTGTTGAAAGAAGCTGCTGAAGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	boltzgen	0.28
tier1_50pmol	SEB_01_All_Generators_boltzgen_binder_008	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGGGTGCTGCTGCTGCTGCTGGTAGAGCTAAAGGTCAAGAAATTAGAGATAAATACGATAAAGAAGCTAACAAATTGGCTAAAGAATTGGAAGGTGAAGAAGCTGATAAAGCTGCTGAAGAATTGGCTGCTGAATACGGTAAAGAAGCTGCTGCTGCTAACGGTAAAATTGGTGAAGCTGCTAAAGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	boltzgen	0.28
tier1_50pmol	SEB_01_All_Generators_boltzgen_binder_005	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGATGATTACTTTGGTTTTCGATGATGGTAAAAAAGTTGAAGTTAAATCTCAAGAAGAATTGTTGGAAGAAATTAAAGTTTTGGGTTTGCCAGAAGAATGCTTGGAATTGACTTACAACGGTAAAACTTTCACTACTTTGGAAGAATTCAAAAAAGTTAAAATTAACGATGGTGATACTGTTAACTTGAAATTGAAATTGCCAGTTGAAGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	boltzgen	0.27
tier1_50pmol	SEB_01_All_Generators_boltzgen_binder_007	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGATGCAAATTTTCGTTAAAACTACTACTGGTAAAACTATTACTTTGGAAGTTGAACCATCTGATACTGTTGGTAACGTTAAAGCTAAAATTCAAGCTATTGAACCATTCGAAGAATTGATTTTCGCTGGTAAACAATTGGAAGATGGTAGAACTTTGTCTGATTACAACATTCAAAAAGAATCTACTTTGCACTTGGTTTTGCCAGGTGAATACGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	boltzgen	0.27
tier1_50pmol	SEB_01_All_Generators_boltzgen_binder_009	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGGCTGCTGGTGCTGCTGCTGGTGCTGCTGGTGGTGCTGCTGGTTCTGCTTCTGCTGCTGGTGCTGGTGCTGGTGCTGGTGGTGGTGAAGGTGGTGCTGCTGGTGCTGCTGCTGGTGGTGCTGCTGGTGCTGCTGCTGGTGCTGCTGCTGGTTCTGCTGCTGCTGCTGCTGGTGCTTTGGGTGAATTGGCTGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	boltzgen	0.26
tier1_50pmol	SEB_01_All_Generators_boltzgen_binder_010	CTAGTGGTGGAGGAGGCTCTGGTGGAGGCGGTAGCGGAGGCGGAGGGTCGGAAAAAAGAGAAAAAATTATTGAATTGTTGTTGGAATTCGGTTTCTCTAAAGAAGAAGCTGAAGAAATTGCTGAAAGATTGTTGGAATTGTACAAAAAAGGTACTTTGAACTTGGCTGCTGTTACTGAATTGTTGGCTGAATTCAAAAAATTGTCTAAAGAAGAAGTTGAAGAAATTTACAAAAGAATTTTGGAAATTTTGTCTGAACAAAAGCTTATTTCTGAAGAGGACTTGTAATAGCTCGAGATCTGATA	boltzgen	0.26