Paraquat · Experiments

Pipeline experiments

Planned and available for reproduction, protocols and order files are included.

TR-FRETAvailableGenerator filter: passed

IVTT TR-FRET screen — Paraquat gBlock constructs

Campaign: paraquat--bg-sm-denovo-khj-e2e · Target slug: Paraquat

96-well IDT gBlocks plate. Construct: T7 promoter + GG + SD + Kozak + ATG + FLAG (DYKDDDDK) + E. coli MFC CDS + TAATAG (2x stop) + GTCGAC + T7 terminator (SOP 03). QC: 20/20 valid per gblock_qc.tsv.

Binders measured

Top WeaveRank

1.15

Generator filter

20/20 passed

1BG_SM_DENOVO_KHJ_E2E_boltzgen_binder_004boltzgen1.15
2BG_SM_DENOVO_KHJ_E2E_boltzgen_binder_012boltzgen0.77
3BG_SM_DENOVO_KHJ_E2E_boltzgen_binder_001boltzgen0.58
4BG_SM_DENOVO_KHJ_E2E_boltzgen_binder_019boltzgen0.49
5BG_SM_DENOVO_KHJ_E2E_boltzgen_binder_016boltzgen0.43

Procedure & files

Vector

linear gBlock template (T7-SD-Kozak-FLAG construct)

Protocols

Materials

IDT gBlocks (96-well plate)
TnT T7 Quick IVTT kit
HTRF anti-FLAG reagents

Conditions

codon_table: E. coli MFC
construct_context: T7+GG+SD+Kozak+ATG+FLAG+CDS+TAATAG+GTCGAC+T7-terminator

Order files

paraquat--bg-sm-denovo-khj-e2e_gblocks_plate.xlsx

Position	Candidate	Sequence	Tool	WeaveRank
A1	BG_SM_DENOVO_KHJ_E2E_boltzgen_binder_004	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGCGATTTATGCGGTGGCGAAAGTGGATTATACCGCGACCCATCCGGATGAACTGAGCTTTAAAAAAGGCGATCTGCTGAAAATTCTGGATGAACCGGATGAAGAATGGTGGCTGGCGCAGAGCCTGACCACCGGCAAAGTGGGCTGGGTGCCGAAAAGCTATGTGGGCGATTTTTTTGAAGAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	1.15
A2	BG_SM_DENOVO_KHJ_E2E_boltzgen_binder_012	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCAGCCTGGAATTTCGTGTGGGCGATCGTGTGTATGCGCGTGTGACCTGGCCGGATGGCAGCGTGGGCTGGTATCCGGCGACCGTGGTGGATATTATTGATGAAGATCGTGTGCTGGTGCGTTTTGATGAAGATGGCACCCAGCGTGTGGTGCATACCGCGGATCTGGTGCCGGCGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.77
A3	BG_SM_DENOVO_KHJ_E2E_boltzgen_binder_001	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCGGCGAAGATGTGCGTGTGGGCAGCCGTGTGTATGCGAAAGCGAGCGATGGCAAATGGTATCCGGCGGAAGTGCTGGCGCTGGAAGGCGATAAAGCGCTGGTGAAATTTCTGGAAAACGGCGAAACCGCGTGGGTGCCGAAAAAAGATCTGCGTGTGGCGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.58
A4	BG_SM_DENOVO_KHJ_E2E_boltzgen_binder_019	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCACCAAAATTGAACTGACCCCGGAACAGCGTGAAAAAGTGCTGGAAGCGTTTCGTAAACTGGATACCGATGGCAGCGGCGTGCTGACCGCGGAAGAACTGAAAGCGGCGCTGAAAGAAGCGGGCCTGAGCGAAGAAACCGCGGATGCGGTGGTGGATGATCTGGGCGAAACCGCGACCCTGGCGCAGGTGGAAGCGTGCCTGGTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.49
A5	BG_SM_DENOVO_KHJ_E2E_boltzgen_binder_016	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCCCGCTGACCGAAGAAGAACGTGAACGTGTGAAAAAAGTGATTGAAGAAGCGATTAAAGAATATGGCCCGGATGCGAGCGCGGAAAAAATTCAGGAATATCTGCGTAGCCTGGGCCTGGATGTGACCGTGGCGGAAATTAAAGAACTGCTGGCGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.43
A6	BG_SM_DENOVO_KHJ_E2E_boltzgen_binder_013	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGATGAAGAACAGCAGCGTATTCTGGATAAAATTAAAGCGCTGAGCGATGAACAGGTGAAAGAACTGGCGAAAGAACTGGGCATTCCGGATGATGAACCGGTGACCCGTGATCTGCTGGTGGAACTGTGCAAAACCAGCGAAAAAAACCTGGAAATTGCGAAAAAATATCTGGAAACCCTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.27
A7	BG_SM_DENOVO_KHJ_E2E_boltzgen_binder_017	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGAAAATTTATGTGAAAGCGCTGAAAGATTATACCGCGAAAACCGCGAACGAACTGAGCTTTAAAAAAGGCGATATTCTGGAAGTGCTGAAAATTCTGGATGATGAATGGTGGGAAGCGCGTAAAGGCGATTATACCGGCCTGATTCCGAGCAAAAACGTGGAAGTGATTAAAGAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.13
A8	BG_SM_DENOVO_KHJ_E2E_boltzgen_binder_007	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGAGCGGCCTGCCGATTGCGCGTGCGCTGGTGGATTATACCGGCAAAGAACCGAACGAACTGAGCTTTAAAAAAGGCGATCTGCTGGAAGTGCTGGGCGTGGAAGGCGATTGGGCGCTGGTGCGTAGCCTGGAAACCGGCGAAGTGGGCTGGGTGCCGATTCGTTATATTGAAATTGTGGCGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.03
A9	BG_SM_DENOVO_KHJ_E2E_boltzgen_binder_008	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGAGCATGACCCCGGAAGAACGTGCGGTGGTGGTGGAAACCATTACCAAAATTGGCGAAAAAGAAAGCGATGAAGAAAAAAAAAAAGCGCTGGGCGAAATTGCGGAAAAAGTGGCGGCGGGCGAAATGACCGCGGAAGAAGCGTGCGAAAAACTGAAAGAAATTGGCCTGCCGGAAGATGCGGAACGTGTGCTGGCGGCGCTGGAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.07
A10	BG_SM_DENOVO_KHJ_E2E_boltzgen_binder_002	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGCGCCGGGCGAACTGGTGCGTGCGCTGAAAGATTATACCGCGACCCGTCCGGATGAACTGAGCTTTAAAAAAGGCGATATTCTGCGTGTGCTGGAAGTGGATCCGGAACATCCGGGCTGGGTGTATGCGCAGAACCTGCAGACCGGCAAAACCGGCTGGATTCCGGCGGATAACGTGGAACCGATTTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.08
A11	BG_SM_DENOVO_KHJ_E2E_boltzgen_binder_006	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCGAAGTGGTGCGTGGCACCGTGACCACCGTGGAACTGGATGGCAAAGAAGTGCCGGCGCTGGAACTGAAAGTGGGCGATGTGGCGCGTGTGGTGGAAGCGGAAGGCGAACTGGAACCGGGCGATATTGTGAAAGTGCTGAAAGTGGAAAAAGATGGCGATAAAGCGCTGGTGGAAGCGGAAGTGCTGGGCCGTGGCCCGGCGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.14
A12	BG_SM_DENOVO_KHJ_E2E_boltzgen_binder_015	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCAAAAAAATTACCAGCGTGAAAGAACTGTATGAAACCGTGAAAAGCAACCCGGGCGCGGTGTTTCGTGCGAAAGCGGATTATGAAGGCAGCGCGCCGGGCGAAGTGCCGTTTAAAAAAGGCGATCTGCTGACCGCGCTGGGCGTGGTGGATGATGATTGGGTGAAAGTGCAGACCAGCAGCGGCCGTGTGGGCTGGGTGAAAGCGGCGGATATTGTGGCGGCGGGCGCGGAACTGCTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.19
B1	BG_SM_DENOVO_KHJ_E2E_boltzgen_binder_020	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGAGCGAAGGCCTGGAACTGGTGAAAAAAGCGATTGAAATTCTGAAAGAACTGCTGGAAAAAGAAACCGATCCGAAAGTGCGTGCGGGCCTGGAAAAAGCGATTGAAATTCTGGAAAAAATGCTGGAAAACAACATTGGCGCGACCAAAGAAGAAATTAAAGAACTGCTGAAAGAATGCGGCCTGCCGGAAGAAATTATTAACGAAATTCTGAGCAAACTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.19
B2	BG_SM_DENOVO_KHJ_E2E_boltzgen_binder_003	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGATGATTCTGACCGCGAAAGAAGTGCTGGGCGAAGGCCCGGAAGGCGAAATTGTGAAATTTGAAGATGGCAGCGTGGGCCTGGTGCTGGAACGTGAAGGCAACATTGCGAAAGCGCGTCTGCTGGAAACCGGCGAAGTGGTGACCGTGAAACTGCCGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.32
B3	BG_SM_DENOVO_KHJ_E2E_boltzgen_binder_010	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGATGATTGTGGTGGCGCTGAAAGATTATGAAGGCGATGAAAACCTGCGTGCGGTGAAAGGCGATACCCTGAAAATTCTGGAAGAAATTGATGATGATCTGTGGAAAGTGGAAAACCTGGAAACCGGCGAAACCTGCCTGGTGCCGAAAAGCCTGATTAAAGAAAAAGAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.34
B4	BG_SM_DENOVO_KHJ_E2E_boltzgen_binder_005	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGATGACCGAAGTGCGTGTGGGCGATCGTGTGATGGCGAAAATTCTGAGCAGCAAAACCGGCAAAACCGAATGGTTTGAAGGCGAAGTGGTGGAAGTGCTGGATGAAGATACCGTGCTGGTGCGTTTTCTGAGCAACGGCGAAGAAGCGGCGGTGCATCGTAAAAACCTGCGTCTGCTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.42
B5	BG_SM_DENOVO_KHJ_E2E_boltzgen_binder_009	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGTGCGAAGGCAAAAAAGTGGTGGCGATTAAAGATTATAAAGCGACCAGCCCGGATGAACTGAGCCTGAAAAAAGGCGAAACCCTGTGCGTGCTGGAAGAACTGGAAGATGGCTGGTGGCTGGCGCAGAGCCTGAGCGATCCGAGCCGTGTGGGCGTGGTGCCGAGCAAATATGTGGAAGAAATTTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.52
B6	BG_SM_DENOVO_KHJ_E2E_boltzgen_binder_018	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGAGCGTGCTGGAAAAACTGAAAGAAATTCTGGAAAAAGTGGAAAGCGGCGAAATTAGCGCGGAAGAAGCGGGCAAAAAAATTGAAGAAGTGACCGGCGTGAAAGTGCCGGAAGATGCGACCAAAGAAGAAATTAAAGAAAAAATTAAAAAAGTGATTAAAGAACTGGAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.54
B7	BG_SM_DENOVO_KHJ_E2E_boltzgen_binder_014	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGCCGGAATTTAAAGTGGGCGATCTGGTGTATGCGAAAGGCGCGGATGGCAAATGGTATCCGGCGAAAGTGGTGAAAGTGGAAGGCGATAAATGCCTGGTGGAATTTCTGGAAAACGGCGAAACCGCGTGGGTGCCGAAAAAAGATCTGAAAAAAGCGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.71
B8	BG_SM_DENOVO_KHJ_E2E_boltzgen_binder_011	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGAGCGCGCGTGTGCGTCGTGCGCGTGCGGCGCTGGCGAGCGTGGGCGCGGAACTGACCGATGCGCAGGTGGCGAAAGTGATTGAAGCGATTGAAGCGAGCCCGGATGGCGGCAAACTGACCGTGGAAGAATTTCGTCATGTGCTGCGTGAAGTGGGCGCGAGCGAAGAAGCGATTGATGCGGTGATTGAACGTTTTCAGGAAGAAGATGGCACCATTAGCCTGGATGATCTGCTGGCGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.72

Yeast Surface DisplayAvailableGenerator filter: passed

Yeast surface display screen — Paraquat binder oligo pools

Campaign: paraquat--bg-sm-denovo-khj-e2e · Target slug: Paraquat

IDT oPools order, tiered by confidence: tier1_50pmol (20 oligos). Gap-repair cloning into pCTCON2.

Binders measured

105

Top Pool score

0.81