Pentachlorophenol · Experiments

Pipeline experiments

Planned and available for reproduction, protocols and order files are included.

TR-FRETAvailableGenerator filter: passed

IVTT TR-FRET screen — Pentachlorophenol gBlock constructs

Campaign: pentachlorophenol--bg-sm-denovo-pci-e2e · Target slug: Pentachlorophenol

96-well IDT gBlocks plate. Construct: T7 promoter + GG + SD + Kozak + ATG + FLAG (DYKDDDDK) + E. coli MFC CDS + TAATAG (2x stop) + GTCGAC + T7 terminator (SOP 03). QC: 20/20 valid per gblock_qc.tsv.

Binders measured

Top WeaveRank

1.35

Generator filter

20/20 passed

1BG_SM_DENOVO_PCI_E2E_boltzgen_binder_001boltzgen1.35
2BG_SM_DENOVO_PCI_E2E_boltzgen_binder_004boltzgen1.09
3BG_SM_DENOVO_PCI_E2E_boltzgen_binder_005boltzgen0.73
4BG_SM_DENOVO_PCI_E2E_boltzgen_binder_002boltzgen0.57
5BG_SM_DENOVO_PCI_E2E_boltzgen_binder_017boltzgen0.51

Procedure & files

Vector

linear gBlock template (T7-SD-Kozak-FLAG construct)

Protocols

Materials

IDT gBlocks (96-well plate)
TnT T7 Quick IVTT kit
HTRF anti-FLAG reagents

Conditions

codon_table: E. coli MFC
construct_context: T7+GG+SD+Kozak+ATG+FLAG+CDS+TAATAG+GTCGAC+T7-terminator

Order files

pentachlorophenol--bg-sm-denovo-pci-e2e_gblocks_plate.xlsx

Position	Candidate	Sequence	Tool	WeaveRank
A1	BG_SM_DENOVO_PCI_E2E_boltzgen_binder_001	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGAGCGAAGAACAGATTGCGGCGGCGGCGGAAGAACTGACCCGTACCAAAGAACTGCTGGATGCGGGCAAAATTACCCTGGAAGAAGCGATTGAACAGCTGAAAAAACTGCATAAACAGGGCCTGACCTATGAAGAACTGGCGCAGGCGATGACCCGTGTGGGCGTGCCGATGACCGCGGAAGAAGTGAAAGAAATGCTGAAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	1.35
A2	BG_SM_DENOVO_PCI_E2E_boltzgen_binder_004	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGATGCCGCTGACCATTAAAATTATTGATGAAAACGGCGAAGTGGTGGCGGAAAAAACCGTGAGCACCGCGGAAGAAGCGCTGGCGTTTGTGCGTGAAGCGCTGAAAATTGTGGGCAACCGTAAAGGCACCGTGATTATTGAAGATGCGGAAGGCAAAGTGCTGGGCACCGTGAGCGGCAAAGCGAGCGAAGAAGGCGCGGAAAAAGTGGCGGCGCTGTGCGGCGAAGCGATGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	1.09
A3	BG_SM_DENOVO_PCI_E2E_boltzgen_binder_005	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGAGCGTGCTGGATCGTGTGGTGGAAGCGGTGCTGGATCTGGATGGCACCCCGCTGCCGGAAGAACTGCAGGCGCTGGTGAAAGGCAAAACCGTGGGCCAGCTGGTGAAAGAAGGCCTGATTGAAATTCTGAGCGTGGATGGCGATGTGGTGGTGGCGCGTGTGCTGGCGGGCCCGCTGGCGGGCAAAGTGGTGCGTCTGATTCTGAAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.73
A4	BG_SM_DENOVO_PCI_E2E_boltzgen_binder_002	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGATGAGCCTGGAAGAAATTCGTAAACTGGTGCAGGAACTGAGCGTGGAAGAACTGGCGGAACGTTGCAAAGAACTGGGCCTGACCGCGGCGGAAGTGCGTGAACGTCTGAAAGCGGTGGGCCTGCCGGAAGAACGTATTGATGAAGTGATTGCGCTGCTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.57
A5	BG_SM_DENOVO_PCI_E2E_boltzgen_binder_017	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGAGCGAAGATACCATTAAACTGAAAGTGGTGACCGAAGATGGCGAAGTGAAAGAAATTGAACTGAACCTGAGCGATACCGTGGCGGAAGCGCGTGAACGTATTAAAGAAGCGACCGGCTATGATACCGCGACCTATAAAGGCGTGGTGCCGCCGGATAGCCTGACCGTGGGCGAAGTGGCGGAAAAATATAACCTGAAAGATAACAGCACCATTACCGTGTTTAAAGGCTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.51
A6	BG_SM_DENOVO_PCI_E2E_boltzgen_binder_003	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGCCGAGCGAAGAACAGCGTGCGGAAGCGCGTAAACTGATTAAAGAAGCGAAAGAAAAAGGCGCGAGCGGCGAAGAACTGCGTAAACTGGCGCGTGAACTGAAAGAACTGGGCCTGACCCCGGAAGAAATTGCGGAAATTTGCACCGAAGTGGGCCTGCCGCGTACCGTGGCGGAAATTAAAGAAATTCTGGAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.12
A7	BG_SM_DENOVO_PCI_E2E_boltzgen_binder_007	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGATGGTGTTTAGCGAAGAACAGGAACGTCTGCTGCGTGAAGCGGTGGAACTGCGTGAAAACGGCGCGAGCGATGCGAAAATTGCGGAAATTGTGCGTAAACTGCGTGAAATGGGCCTGAGCGCGGAAGAAATTGCGGAAGCGGCGACCGAACTGGGCTGCCCGGTGACCGCGGCGGAAATTGATGAACTGCTGAAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.11
A8	BG_SM_DENOVO_PCI_E2E_boltzgen_binder_012	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCGAAGAACTGAAAGCGGCGGCGATTGCGGCGGTGCGTGCGGCGGCGGATGCGGATGGCTATGTGAGCCGTGCGCAGCTGATTGCGGCGCTGGAAGCGGCGGGCATGGATGCGGAAAAAGCGGCGGAACATTGGGAAGAACTGGGCAAAGAACGTGTGACCGTGGCGGAAGCGGAAGAAATTCTGAACAGCATGAGCGATGAAGAACTGGAAAGCCTGGTGAAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.11
A9	BG_SM_DENOVO_PCI_E2E_boltzgen_binder_006	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGCGATTGTGACCGCGCAGATTCTGAGCGGCCCGGTGCCGAAAGTGGGCGATATTCTGGAAATTCTGGAAGTGGATGGCGATATTGCGGTGGCGCAGAGCCTGCAGACCGGCGCGGTGGTGGTGGCGCGTATTCTGAGCGGCGAACTGCCGAAAGTGGGCGATAAAGTGCGTATTACCGAAGTGGATGAAGAAACCGGCGTGGTGACCGGCGAAATTATTTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.09
A10	BG_SM_DENOVO_PCI_E2E_boltzgen_binder_019	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCAAAAAAAAATATAAAGTGAAAGTGTATGATGCGGAAACCGGCGAAGTGATTGGCGAAGGCGAAATTGAAGCGGAAAGCGAAGAAGAAGCGAAAGAAAAAGCGAAACGTATTGCGAAAGAAAAAACCGGCGTGAAAATTGGCAAAATTGAAGTGGAAGAAGTGGAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	0.01
A11	BG_SM_DENOVO_PCI_E2E_boltzgen_binder_013	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGCGACCCCGGAACAGCTGGAACGTGCGGTGGCGCTGGGCCGTGAAGCGCTGGCGAAACATGGCAAAAGCCCGAGCGTGGAAGAAGCGGCGGAAGTGGGCCGTCGTTGCAAAGAACTGGGCCTGACCATTGAAGATGCGGAAGAAGCGATGAAAGAAGCGGGCGCGGAAGATGCGAGCCCGGAAGCGCGTGAAGCGGCGTATGAAGCGCTGAAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.13
A12	BG_SM_DENOVO_PCI_E2E_boltzgen_binder_009	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCGAACTGGAACGTCGTGTGGAAGAAGCGGCGAAACTGAGCGAAGAAGAAATTCGTGAACGTCTGAGCAAACTGAGCGAAGAAGAACTGAAAGAAGCGCTGAAAGTGGCGAAAAAACTGGGCCATGAACGTGTGGTGGAAATTATTGAAAAACTGCTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.26
B1	BG_SM_DENOVO_PCI_E2E_boltzgen_binder_011	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGCGCTGACCGAAGAACAGGAAGCGGCGCTGCGTGAAGCGGTGGAACTGGTGCGTGAAAAAGGCCCGGATGATCCGGAAGCGCAGGCGGTGGGCACCAAAGCGGTGCGTCTGATGAAAGAAGCGGGCTTTACCGCGGAAGAAATTGCGGCGGCGTTTACCGCGTATGGCCTGCCGGCGACCGTGGCGCAGGTGGAAGAACTGCTGAAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.30
B2	BG_SM_DENOVO_PCI_E2E_boltzgen_binder_016	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGATGACCCCGGAAGAACGTGAAGAACTGCGTCGTCGTGCGCTGGAAGAAGCGCGTCGTAAAATTGAAGCGGGCGAAATTACCGCGGAAGAAGCGGCGCGTAAACTGGAAGATCTGGGCATTGTGAGCGAAGAAGAAGCGCTGGCGGCGCTGGCGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.37
B3	BG_SM_DENOVO_PCI_E2E_boltzgen_binder_008	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGAGCATTCGTGTGGGCGATGTGGTGCTGGCGACCCTGCCGGATGGCCGTACCCTGCGTGCGACCGTGGTGGAAATTCATGAAGATGGCACCGCGGTGGTGAAATTTGATGAAACCGGCGAAGAAGCGGTGGTGCCGCTGAGCCGTCTGCGTCCGGTGAGCTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.50
B4	BG_SM_DENOVO_PCI_E2E_boltzgen_binder_010	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCCTGGCGCGTCGTGCGGCGATTCTGGGCGGCAAAGCGAACCCGGAACTGGCGAAAAAAGCGCTGGAAGCGGTGAAAGCGGGCGATGTGGAAAAAGCGGTGGCGCTGGGCCGTGAAGCGGCGAAAGCGGGCGGCGCGAGCGAAGAAGAAATTGAAGCGGCGGCGGAAGTGGTGCTGGAAGATCCGGAAAAAGCGATTGAACTGCTGAAAGAAGGCCTGGCGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.62
B5	BG_SM_DENOVO_PCI_E2E_boltzgen_binder_015	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGCGGCGGTGCGTGTGGGCGATCGTGTGCTGGCGAAACTGCCGGATGGCAAATGGGTGCCGGCGGTGGTGAAAGCGGTGGATGAAGAACGTGCGCTGGTGCAGTTTCTGAGCAACGGCAAAGAAGCGTGGGTGCCGCTGAAAGATCTGCGTCGTGCGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.64
B6	BG_SM_DENOVO_PCI_E2E_boltzgen_binder_014	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGAGCATTGCGGTGGGCGATGAAGTGCTGGCGAAAGCGCCGGATGGCAAATGGCTGAAAGGCGTGGTGAAAAAAATTGAAGGCGATAAAGCGCTGGTGCGTTTTCTGGAAACCGGCGAAGAAGCGTGGGTGCCGCTGAAAGATCTGAAAGTGGTGTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.64
B7	BG_SM_DENOVO_PCI_E2E_boltzgen_binder_018	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGAGCCCGGTGGAAGAACGTGCGATTGCGCTGGCGAAAGCGGGCCGTCCGGAAGAAGCGGTGCGTCTGCTGGCGAAAGCGGGCTATACCGAAGAAGAAATTGCGCGTATTCTGGCGAAAGCGGGCGTGAGCGCGACCGTGGCGCAGATTCGTGAAGCGGTGAAATAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.71
B8	BG_SM_DENOVO_PCI_E2E_boltzgen_binder_020	TAATACGACTCACTATAGGGGGAGGAGGGCCACCATGGACTACAAAGACGATGACGACAAGGGCCCGACCGATAGCCTGACCGAAGAACAGAAAAAAACCGTGATTGAAGTGGCGAAAAAACAGCTGGAAGAAGGCATTACCGAACTGAGCCGTGAAGAAGCGATTGAACTGCTGAAAGAAGCGGGCCTGAGCGAAGAAGATGCGGAACGTGCGGCGAAACTGCTGGAAGAACTGGGCAGCCTGACCAAAGCGATGGAAGAAGTGAGCTAATAGGTCGACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG	boltzgen	-0.98

Yeast Surface DisplayAvailableGenerator filter: passed

Yeast surface display screen — Pentachlorophenol binder oligo pools

Campaign: pentachlorophenol--bg-sm-denovo-pci-e2e · Target slug: Pentachlorophenol

IDT oPools order, tiered by confidence: tier1_50pmol (20 oligos). Gap-repair cloning into pCTCON2.

Binders measured

109

Top Pool score

0.88